10 mL media
1 mL Alamar Blue (found in left normal Freezer; changes color of media to red/pink)
1. Mix Alamar Blue and Media together in a trough.
2. Aspirate old media from 96-well plate.
3. Add 100ul of Alamar Blue/Media Solution to each well.
4. Incubate for 4 hours.
5. Read on plate reader
a. Ensure the bulb is warmed up
b. Click on Re-read
c. Save and Export results to Excel
d. Title with Date, Initial and MTT.