Alamar Blue


10 mL media
1 mL Alamar Blue (found in left normal Freezer; changes color of media to red/pink) 

1.  Mix Alamar Blue and Media together in a trough.
2.  Aspirate old media from 96-well plate.
3.  Add 100ul of Alamar Blue/Media Solution to each well.
4.  Incubate for 4 hours.
5.  Read on plate reader
a.  Ensure the bulb is warmed up
b.  Click on Re-read
c.  Save and Export results to Excel
d.  Title with Date, Initial and MTT.