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1% Agarose Gel

For 250 mL:

5 mL 50x TAE

2.5 g. agar

245 mL ddH2O

Microwave 3 min. until clear. Can store extra in bottle.

 

Gel Buffer Running Solution

 

750 mL ddH2O

15 mL 50x TAE

20 uL Ethidium Bromide

 

Small Gel

250 mL ddH2O

5 mL 50x TAE

6.5 uL Ethidium Bromide

 

10x Ligation Buffer

 

[Final]

0.5 M Tris, pH 7.5

100 mM MgCl2

100 mM DTT

0.5 mg/mL BSA

10 mM ATP

5 mM Spermidine

 

BSA 10x Buffer for DRA III

 

10 uL BSA

90 uL ddH2O

Vortex; place 1 uL per sample.