LB Derivations

 

LB Broth

For 2 Liters:

20 g. tryptone

10 g. yeast extract

20 g. NaCl

q.s. to 2 L w/ ddH2O

Adjust pH to 7.4 w/ NaOH

Autoclave 45 min.

Divide into 250 mL aliquots

 

LB Plates

500 mL LB broth (pH 7.4)

7.5 g. Noble Agar (15 g/L)

Divide into 2- 250 mL bottles

Autoclave 45 min.

Cool to 55oC in H2O bath

Add 500 uL ampicillin

Pour plates, store stacked. Good for 1 month.