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Lower Gel Buffer (LGB 4x)

90.9 g. 1.5 M Tris

2 g. 0.4% SDS

Adjust pH to 8.8

q.s. to 500 mL w/ ddH2O

 

Upper Gel Buffer (UGB 4x)

30.3 g. 0.5 M Tris

2 g. 0.4% SDS

Adjust pH to 6.8

q.s. to 500 mL w/ ddH2O

 

30% Acrylamide

150 g. 29.2% Acrylamide

4.1 g. 0.8% Bis-Acrylamide

q.s. to 513.5 mL w/ ddH2O

Filter sterilize; store at 4oC; store in opaque/dark bottle.